Journal: PLoS ONE

Article Title: Emulsified Phosphatidylserine, Simple and Effective Peptide Carrier for Induction of Potent Epitope-Specific T Cell Responses

doi: 10.1371/journal.pone.0060068

Figure Lengend Snippet: (A) PS-conjugated NP 366–374 (A/PR8) peptide, PS-conjugated OVA 257–264 peptide or unconjugated peptide was inoculated into B6 mice (3 to 4 mice per group). The in vivo cytotoxicity assay was performed as described in . *p<0.01, **p<0.0001. (B) Splenocytes from naïve mice and mice immunized with PS-conjugated or unconjugated peptide in the presence of poly(I:C) were stained with tetramer and anti-mouse CD8 Ab. The percentage indicates the tetramer-positive cells in total CD8 + cells. The experiment was repeated three times with similar results.

Article Snippet: CD8 + cells from the spleens of B6 mice immunized with PS-conjugated NP 366–374 (A/PR8) peptide plus poly(I:C) were positively selected with BD Imag™ anti-mouse CD8 magnetic particles (BD Biosciences) to achieve >99% purity.

Techniques: In Vivo, Cytotoxicity Assay, Staining